Nucleic Acid Extraction Kit (A02)
Detection principle
After releasing the genomic DNA by splitting cells with the lysis buffer, the magnetic bead could selectively bind to the genomic DNA in the sample. A small number of impurities that are absorbed by the magnetic bead could be removed by the wash buffer. In TE, the magnetic bead could release the boundgenome DNA, obtaining the high-quality genome DNA. This method is simple and quick and the extracted DNA quality is high, which could meet the requirement for the detection of DNA methylation. Meanwhile, the extraction kit based on the magnetic bead could be compatible with automatic nucleic acid extraction, meeting the high-throughput nucleic acid extraction tasks.
Main components of the reagent
The components are shown in table 1:
Table 1 Reagent Components and Loading
|
Component name |
Main components |
Size (48) |
Size (200) |
|
1. Digestion Buffer A |
Tris, SDS |
15.8 mL/bottle |
66mL/bottle |
|
2. Lysis Buffer L |
Guanidinium Isothiocyanate, Tris |
15.8 mL/bottle |
66mL/bottle |
|
3. Wash Buffer A |
NaCl, Tris |
11 mL/bottle |
44 mL/bottle |
|
4. Wash Buffer B |
NaCl, Tris |
13 mL/bottle |
26.5mL/bottle *2 |
|
5. TE |
Tris, EDTA |
12 mL/bottle |
44 mL/bottle |
|
6. Protease K solution |
Protease K |
1.1mL/piece |
4.4mL/piece |
|
7. Magnetic bead suspension 2 |
Magnetic beads |
0.5mL/piece |
2.2mL/piece |
|
8. Instructions to extracting nucleic acid reagents |
/ |
1 copy |
1 copy |
Components that are required in the nucleic acid extraction, but are not included in the kit:
1. Reagent: Anhydrous ethanol, isopropanol, and PBS;
2. Consumables: 50mL centrifuge tube and1.5mL EP tube;
3. Equipment: Water bath, pipettes , magnetic shelf, centrifuge, 96-well plate (automatic), automatic nucleic acid extraction equipment (automatic).
Basic information
Sample requirements:
1.The detection shall be completed under the 7-day storage of ambient temperature after the collection of the cervical exfoliated cell sample (non-fixed).
2.The detection shall be completed under the 30-day storage of ambient temperature after the collection of the cervical exfoliated cell sample (fixed)..
3.The detection shall be completed under the 30-day storage of ambient temperature after the collection of the urine specimen; The detection shall be completed in time after the collection of the cultured cell samples.
Parking specification: 200 pcs/box, 48 pcs/box.
Storage conditions: 2-30℃
Period of validity: 12 months
Applicable device: Tianlong NP968-C nucleic acid extraction instrument, Tiangen TGuide S96 nucleic acid extraction instrument, GENE DIAN EB-1000 nucleic acid extraction instrument.
Medical device record certificate No./product technical requirement No.: HJXB No. 20210100.
Date of approval and revision of the instructions: Approval date: Nov. 18, 2021
About Us
As a high-tech enterprise founded in 2018 by top epigenetic experts, Epiprobe focuses on the molecular diagnosis of cancer DNA methylation and precision theranostics industry. With a profound technology basis, we aim to lead the era of new products to nip cancer in the bud!
Based on Epiprobe core team’s long-term research, development and transformation in the field of DNA methylation with the cutting-edge innovations, combined with the unique DNA methylation targets of cancers, we use a unique multivariate algorithm combining big data and artificial intelligence technology to independently develop an exclusive patent-protected liquid biopsy technology. By analyzing the methylation level of specific sites of free DNA fragments in the sample, the shortcomings of traditional examination methods and the limitations of surgery and puncture sampling are avoided, which not only achieves accurate detection of early cancers, but also enables real-time monitoring of cancer occurrence and development dynamics.
